How to Perform Kaplan-Meier Survival Analysis in GraphPad Prism

TL;DR

To perform a Kaplan-Meier survival analysis in GraphPad Prism, enter your survival data into a compatible table, specifying time and survival status for each subject. The software automatically generates Kaplan-Meier curves to visualize survival differences and allows for statistical comparisons using tests like chi-square and log-rank.

Transcript

hello I'm James Clark from King's College London and in this short video I'm going to show you how to carry out survival analysis in graphpad prism in order to carry out survival analysis and plot a kaplan-meier curve and look at survival differences between groups you need to enter your data into a table compatible with survival analysis prism off... Read More

Key Insights

• 👤 GraphPad Prism provides a user-friendly interface for conducting survival analysis and generating Kaplan-Meier curves.
• 🤨 Survival data is entered into a table, with each row representing a subject and each column representing time or treatment group.
• 👻 Kaplan-Meier curves can be customized in multiple ways, allowing for clear visualization of survival differences.
• 🏆 GraphPad Prism also includes statistical tests such as chi-square, log-rank, and Gehan-Breslow-Wilcoxon tests to compare survival between groups.
• 🥳 The software can compute hazard ratios and 95% confidence intervals to provide additional insights into the data.
• 👥 Pairwise comparisons between different groups can be performed to assess specific group differences.

Questions & Answers

Q: How do you enter survival data in GraphPad Prism?

Survival data is entered into a table, where each row represents a subject and each column represents the time elapsed or treatment group. For each observation, the time is entered in the X column, and 1 or 0 is entered in the Y column based on whether the subject died or not.

Q: Can GraphPad Prism handle datasets with different start and end dates for each subject?

Yes, GraphPad Prism allows the entry of start and end dates for subjects with different durations of study. This is useful when comparing survival across groups where subjects entered the study at different times.

Q: How does GraphPad Prism calculate the survival data?

Once the survival data is entered, GraphPad Prism automatically calculates the percentage survival for each time point. The survival data can be accessed in the results section of the software.

Q: How can Kaplan-Meier curves be customized in GraphPad Prism?

GraphPad Prism offers various customization options for Kaplan-Meier curves. These include choosing different ways to join data points, adding error bars or confidence intervals, and displaying censored data points. The graph can be further customized by changing the graph type and adjusting the appearance of the curve.

Summary & Key Takeaways

• The video demonstrates how to enter survival data into a table compatible with GraphPad Prism for analysis.

• It explains the process of entering data for each subject, including the time elapsed and their survival status.

• The video also shows how to visualize the survival data using Kaplan-Meier curves and customize the graph.