Determining aflatoxins in raw peanuts using immunoaffinity column as sample clean-up method followed by normal-phase HPLC-FLD analysis
https://www.sciencedirect.com/science/article/pii/S0956713522002584
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Summary
This study developed a simplified method for analyzing aflatoxins in raw peanuts using an immunoaffinity column (IAC) and normal-phase HPLC-FLD analysis. The proposed mobile phase improved the fluorescence signal without the need for derivatization. The miscibility of the eluate in the normal-phase solvent was improved by using acetonitrile as the eluent and mixing with toluene. The method eliminated time-consuming procedures and improved recovery and repeatability. The LOQ was as low as 2.0-0.5-1.0-0.5 ng/g of aflatoxin B1-B2-G1-G2. The method outperformed the official method and met FDA standards. The proposed mobile phase had a prolonged shelf-life and required less organic solvent.
Top Highlights
- This study was aimed at simplifying the conventional aflatoxin HPLC-FLD analysis procedure for determining aflatoxins B1, B2, G1, and G2 by utilizing the advantage of immunoaffinity column (IAC) and normal-phase chromatography. Normally, a derivatization procedure is needed to enhance the fluorescence signal of aflatoxin quenched by the reversed-ph...
- Aflatoxins (AF) have been identified as carcinogens and have many adverse health effects on humans and animals,
- high-performance liquid chromatography (HPLC) combined with a fluorescence detector (FLD) is widely used because it has been applied in many official methods (Cai et al., 2020). Also, the cost for the equipment and the maintenance for HPLC-FLD are affordable to most labs.
- Sample extraction uses organic solvents to extract AF from the sample which has been broken down into small particles
- Chloroform was widely used before the prevalence of immunoaffinity column (IAC) as a extraction method since it has a high AF recovery rate (Möller & Nyberg, 2004). Nonetheless, chloroform is highly toxic, which does not fulfill environmental-friendly requirements nowadays