Expression and purification of His-tagged proteins from E. coli

Transcript

hello protein purifiers in this video i'm going to show you how to recompently express a his tagged protein in e coli and purify using immobilized metal affinity chromatography the first thing that we're going to need to do is make some media for e coli cultures this would typically be lb media or you could use 2yt media or terrific broth depending... Read More