Improve the Quality of your Sorted Cells with Microfluidic Technology

TL;DR

Flow shift cell sorting is a gentler alternative to conventional cell sorting techniques, reducing cellular stress and improving cell quality.

Transcript

for decades biologists have used flow cytometry based cell sorting to isolate cells from complex samples like blood or serum this involves labeling the cells of interest with antibodies before introducing them along with any unlabeled cells into a cell sorter via a nozzle as the cells enter the sorter they are injected into a stream of sheath fluid... Read More

Key Insights

• ❓ Conventional cell sorting techniques can cause cellular stress, hindering the reliable and reproducible downstream studies.
• ❓ Neurons, sperm cells, spheroids, and undifferentiated cells are particularly sensitive to the damaging effects of conventional cell sorting techniques.
• 👨‍🔬 Flow shift cell sorting eliminates damaging steps, reducing cellular stress and providing better quality cells for various research applications.
• 😒 The use of a small disposable microfluidic chip in flow shift cell sorting reduces the risk of contamination and enhances data quality.
• 👻 Flow shift cell sorting allows customization of sheath fluid, providing a consistent environment for target cell types.
• 👨‍🔬 The technology of flow shift cell sorting holds great potential in advancing research fields beyond immune cell studies, such as neuroscience and reproductive research.
• 💐 Flow shift cell sorting can successfully remove rare undifferentiated cells from induced pluripotent stem cell cultures, which is essential to prevent tumor development.

Questions & Answers

Q: What is the main problem with conventional electrostatic droplet-based cell sorting?

Conventional cell sorting techniques can cause cellular stress, leading to delayed cell growth, functional changes, and even cell death due to high pressure, electric charge, and frequent collisions within the system.

Q: What are the limitations of conventional cell sorting techniques in neuroscience research?

While conventional sorting techniques have little impact on neuron viability, they can hinder neurite outgrowth, limiting their usefulness in neuroscience research.

Q: How does flow shift cell sorting overcome the limitations of conventional techniques?

Flow shift cell sorting eliminates damaging steps involved in conventional cell sorting, reducing cellular stress in various cell types, including neurons, sperm cells, spheroids, and undifferentiated cells.

Q: What advantages does flow shift cell sorting offer over conventional techniques?

Flow shift cell sorting utilizes a small disposable microfluidic chip, reducing the risk of contamination and providing a consistent environment for target cell types. It also allows customization of sheath fluid for specific cell types and produces better quality cells for a broader range of research applications.

Key Insights:

• Conventional cell sorting techniques can cause cellular stress, hindering the reliable and reproducible downstream studies.
• Neurons, sperm cells, spheroids, and undifferentiated cells are particularly sensitive to the damaging effects of conventional cell sorting techniques.
• Flow shift cell sorting eliminates damaging steps, reducing cellular stress and providing better quality cells for various research applications.
• The use of a small disposable microfluidic chip in flow shift cell sorting reduces the risk of contamination and enhances data quality.
• Flow shift cell sorting allows customization of sheath fluid, providing a consistent environment for target cell types.
• The technology of flow shift cell sorting holds great potential in advancing research fields beyond immune cell studies, such as neuroscience and reproductive research.
• Flow shift cell sorting can successfully remove rare undifferentiated cells from induced pluripotent stem cell cultures, which is essential to prevent tumor development.
• Flow shift cell sorting improves the quality of cells for a broader range of research applications, making it a promising alternative to conventional techniques.

Summary & Key Takeaways

• Flow cytometry-based cell sorting is commonly used to isolate cells from complex samples using antibodies and high-pressure fluid streams.

• Conventional cell sorting methods can cause cellular stress, leading to hindered cell growth, changes in functionality, and even cell death.

• Flow shift cell sorting, a new technique, eliminates damaging steps and reduces cellular stress, resulting in better quality cells for a broader range of research applications.