Genetically encoded reporters of protein kinase A activity reveal
 impact of substrate tethering | Proceedings of the National Academy of Sciences thumbnail
Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering | Proceedings of the National Academy of Sciences
www.pnas.org
When AKAR1 was transfected into HeLa cells, the fluorescence was uniformly distributed in the cytosolic compartment but excluded from the nucleus (Fig. 3A, leftmost frame), as expected for an 82-kDa protein without targeting signals. A similar expression pattern was observed in COS-7, HEK293, and Ch
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  • When AKAR1 was transfected into HeLa cells, the fluorescence was uniformly distributed in the cytosolic compartment but excluded from the nucleus (Fig. 3A, leftmost frame), as expected for an 82-kDa protein without targeting signals. A similar expression pattern was observed in COS-7, HEK293, and Chinese hamster ovary cells (data not shown). The re...

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