Long-term in vivo recording of circadian rhythms in brains of freely moving mice | PNAS thumbnail
Long-term in vivo recording of circadian rhythms in brains of freely moving mice | PNAS
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Using this system, we demonstrate the dynamics of Per2 and Cry1 transcriptional rhythms in SCN vasoactive intestinal peptide (VIP) neurons following an 8-h phase advance in the light/dark cycle. the reporter we designed contains both the Cry1 promoter and the intron. To maintain the signal in the ce
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  • Using this system, we demonstrate the dynamics of Per2 and Cry1 transcriptional rhythms in SCN vasoactive intestinal peptide (VIP) neurons following an 8-h phase advance in the light/dark cycle.
  • the reporter we designed contains both the Cry1 promoter and the intron.
  • To maintain the signal in the cell body and to increase turnover kinetics (18, 19), a nuclear localization signal (NLS) and residues 422 to 461 of mouse ornithine decarboxylase (D2),

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