F-actin and Myo8 are not required for gametosome formation.
The final division plane angles of classified cells were compared with control cells (n = 5) using t test. ***P < 0.02; ns, P > 0.5. Illustration of the spindle angle (within 30° or over 30°) on the right of graph. The dotted line shows 30°, and the gray structure indicates the spindle.
Actin inhibition has little impact on division plane determination of the gametophore initial division. (A) Localization of actin filaments during the first gametophore division visualized by the Lifeact-GFP marker. No twin peaks pattern or accumulation at the presumed gametosome location could be observe
Effectiveness of latrunculin B was confirmed with the treatment of the Lifeact-GFP line.
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