DNA cloning: A personal view after 40 years | PNAS
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unrelated bacterial species can be combined and propagated using the same approach Supporting the notion that DNA was unlikely to survive in cells of an unrelated species was the finding that individual biological species maintain characteristic ratios of A+T to G+C base pairs “Construction of Biolo
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- unrelated bacterial species can be combined and propagated using the same approach
- Supporting the notion that DNA was unlikely to survive in cells of an unrelated species was the finding that individual biological species maintain characteristic ratios of A+T to G+C base pairs
- “Construction of Biologically Functional Bacterial Plasmids In Vitro,” my colleagues A. C. Y. Chang, H. W. Boyer, R. B. Helling, and I reported in November 1973
- genes from eukaryotic cells can be cloned in bacteria
- Bacterial viruses and plasmids had been shown to pick up DNA from the chromosomes of their hosts
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