To determine whether the circadian clock regulates the host response to infection with S. Typhimurium, we infected wild-type (WT) mice by oral gavage either at 10:00 AM (day, early rest phase; zeitgeber time 4, ZT4) or at 10:00 PM (night, early active phase; ZT16). At 48, 60, 72, and 78 h postinfection (p.i.), mice were killed, and tissue samples c...
mice infected during the day showed increased expression of these genes compared with uninfected controls, and the expression was dependent both on time of infection and time of death (Fig. 1D).
Genes in mice infected in the morning (ZT4) or at night (ZT16) showed maximal expression when killed during the day (48 and 72 h for mice infected at ZT4; 60 h for mice infected at ZT16).
Altogether, these results suggest that a circadian mechanism regulates components of the host response upon Salmonella infection and that clock disruption largely affects this response.
Moreover, the time-dependent changes observed in the transcription of genes involved in dendritic cell development and leukocyte function, as well as other immune genes included in cluster 4 (Fig. 4A and Dataset S1), suggest a possible circadian control of the development of innate and adaptive immunity following infection.
Glasp is a social web highlighter that people can highlight and organize quotes and thoughts from the web, and access other like-minded people’s learning.