A fluorophore ligase for site-specific protein labeling inside living cells | PNAS thumbnail
A fluorophore ligase for site-specific protein labeling inside living cells | PNAS
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fluorophore ligation occurs in cells in 10 min and that it is highly specific for LAP fusion proteins over all endogenous mammalian proteins Ligated azide could then be chemoselectively derivatized using cyclooctyne-fluorophore conjugates. , we created a ligase capable of directly attaching the blue
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  • fluorophore ligation occurs in cells in 10 min and that it is highly specific for LAP fusion proteins over all endogenous mammalian proteins
  • Ligated azide could then be chemoselectively derivatized using cyclooctyne-fluorophore conjugates.
  • , we created a ligase capable of directly attaching the blue fluorophore 7-hydroxycoumarin to proteins fused to a 13-amino acid LplA recognition sequence
  • LplA’s natural function is to ligate lipoic acid onto three E. coli proteins involved in oxidative metabolism

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