Efficient CRISPR-mediated mutagenesis in primary immune cells using CrispRGold and a C57BL/6 Cas9 transgenic mouse line thumbnail
Efficient CRISPR-mediated mutagenesis in primary immune cells using CrispRGold and a C57BL/6 Cas9 transgenic mouse line
www.pnas.org
Significance Efficient clustered regularly interspaced short palindromic re- peats (CRISPR)/CRISPR associated protein 9 (Cas9)-mediated mutagenesis is necessary for robust genetic screening in pri- mary cells and requires sufficiently high levels of Cas9 and re- liable single guide RNAs (sgRNAs). We
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  • Significance Efficient clustered regularly interspaced short palindromic re- peats (CRISPR)/CRISPR associated protein 9 (Cas9)-mediated mutagenesis is necessary for robust genetic screening in pri- mary cells and requires sufficiently high levels of Cas9 and re- liable single guide RNAs (sgRNAs). We provide a sgRNA design tool that selects high-fid...

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