The bound vesicles were eluted by incubating with HRV-3C protease at a final concentration of ~0.014 mg/mL at 4 °C for 2 h.
Dounce homogenizer for ~30 to 60 strokes
We added EDTA to potentially reduce divalent ion–mediated vesicle and protein aggregation, but we have not tested whether incorporating EDTA is necessary.
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