In an initial attempt to generate loss-of-function mutations using CRISPR/Cas9 in corals, we targeted the Acropora millepora genes encoding fibroblast growth factor 1a (FGF1a), green fluorescent protein (GFP), and red fluorescent protein (RFP). FGF1a appears to be single copy in the genome and encodes an extracellular FGF-signaling ligand; it was c...
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