Roots were then treated with 5 mM nitrate to mimic a nitrogen influx (7).
The growing conditions and nitrogen treatments were selected based on known nitrogen responses and tested treatment regimes that best preserved nitrogen responses during protoplast-generating treatment (supporting information (SI) Text).
Root architecture is highly plastic in response to different levels of nitrogen
For example, responses that occurred in pericycle founder cells are particularly interesting, because these cells will eventually mediate a dramatic change in root architecture under long-term exposure to treatment conditions (Fig. 3).
changes in lateral root regulation had occurred because PLETHORA1 (PLT1), which is expressed discretely in initiating lateral roots within the pericycle (14, 15), was sharply up-regulated in pericycle founder cells (Fig. 1a, cluster 12).
Glasp is a social web highlighter that people can highlight and organize quotes and thoughts from the web, and access other like-minded people’s learning.