Histopathological Differential Diagnosis of Meningoencephalitis in Cetaceans: Morbillivirus, Herpesvirus, Toxoplasma gondii, Brucella sp., and Nasitrema sp.
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For Nasitrema spp. detection, primer sets (5′-CGGATTGGTTTTCGTTGTCT-3′ and 5′-ACCCAACCTAAGCAAGAGCA-3′) were generated and designed based upon the partial NADH dehydrogenase subunit 3 gene of Nasitrema delphini. (GenBank Accession no. KT180216), amplifying a fragment of approximately 230 bp, using Pri
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- For Nasitrema spp. detection, primer sets (5′-CGGATTGGTTTTCGTTGTCT-3′ and 5′-ACCCAACCTAAGCAAGAGCA-3′) were generated and designed based upon the partial NADH dehydrogenase subunit 3 gene of Nasitrema delphini. (GenBank Accession no. KT180216), amplifying a fragment of approximately 230 bp, using Primer3 software (http://bioinfo.ut.ee/prime~r3-0.4.0...
- 0.5 g of fresh-frozen brain cortex (frontal lobe) from each animal was mechanically macerated in lysis buffer and subsequently centrifuged. DNA/RNA extraction was carried out from each 300 μL macerated sample by pressure filtration method, using a QuickGene® Mini 80 nucleid acid isolation instrument, using the DNA Tissue Kit S (QuickGene, Kurabo, J...
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