After 48 h (day 2), the cells were cultured in maintenance medium with 0.5 µM rosiglitazone and 20 µM Rb1. After an additional 48 h (day 4), the medium was changed to fresh maintenance medium with 1 µM rosiglitazone for additional 2 days. At 6 days, cells are fully differentiated to mature beige adipocytes.
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