Recent Advances in Strategies for the Cloning of Natural Product Biosynthetic Gene Clusters
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Cosmid vectors can accommodate up to approximately 40 kb of DNA. They are multicopy plasmids in E. coli that facilitate DNA isolation and in vitro manipulation. However, loss of inserted DNA sometimes occurs within cosmid clones, which may be indicative of sequences that are instability in E. coli o
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- Cosmid vectors can accommodate up to approximately 40 kb of DNA. They are multicopy plasmids in E. coli that facilitate DNA isolation and in vitro manipulation. However, loss of inserted DNA sometimes occurs within cosmid clones, which may be indicative of sequences that are instability in E. coli or of the transcription/translation products of the...
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