Prevalence of errors in lab-made plasmids across the globe
www.biorxiv.org
We show that nearly half of them contained design and/or sequence errors. For transfer plasmids used in making AAV vectors, which are widely used in gene therapy, about 40% carried mutations in ITR regions due to their inherent instability, which is influenced by flanking GC content.
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- We show that nearly half of them contained design and/or sequence errors. For transfer plasmids used in making AAV vectors, which are widely used in gene therapy, about 40% carried mutations in ITR regions due to their inherent instability, which is influenced by flanking GC content.
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