Highly multiplexed design of an allosteric transcription factor to sense novel ligands
www.biorxiv.org
s. We used FuncLib to generate a library of TtgR variants in a ligand-agnostic manner (i.e., not targeted toward any particular ligand) w We carried out secondary and tertiary screens We observed remarkable diversity in the specificity profiles of different variants Quinine and naltrexone have uniqu
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- s. We used FuncLib to generate a library of TtgR variants in a ligand-agnostic manner (i.e., not targeted toward any particular ligand) w
- We carried out secondary and tertiary screens
- We observed remarkable diversity in the specificity profiles of different variants
- Quinine and naltrexone have unique cluster profiles due to their distinct structures
- The hydroxylated tamoxifen derivatives, 4-hydroxytamoxifen, and endoxifen, share similar top-performing cluster
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