Scx expression begins in activated SCs and continues throughout regenerative myogenesis after injury.
By SC-specific Scx gene inactivation (ScxcKO), we show that Scx function is required for SC expansion/renewal and robust new myofiber formation after injury.
In adult muscles, Hic1+ quiescent mesenchymal progenitors (MPs) give rise to Scx+ cells in the muscle interstitial compartment, and ablation of Hic1+ cells negatively impacts muscle regeneration18.
Muscle interstitial Scx+ cells engrafted into the muscle contribute only to extracellular matrix remodeling19. A survey of muscle interstitial CT assigned a sub-population of cells expressing tendon markers (including Scx) as paramysial cells - cells lining next to the perimysium that wraps around muscle fascicles20. Furthermore, Strenzke and colle...
we utilized ScxCreERT2 for tamoxifen (TMX) inducible lineage tracing with a tdTomato (tdT) reporter (RtdT)24. Two experimental groups with different tamoxifen (TMX) and injury regimen were designed (Fig. 2A): 1) TMX-induced marking before injury, and 2) TMX-induced marking after injury; muscles were harvested at 14 dpi for analysis.
Glasp is a social web highlighter that people can highlight and organize quotes and thoughts from the web, and access other like-minded people’s learning.