Skeletal Muscle Satellite Cells Co-Opt the Tenogenic Gene Scleraxis to Instruct Regeneration - 2023.12.10.570982v1.full.pdf thumbnail
Skeletal Muscle Satellite Cells Co-Opt the Tenogenic Gene Scleraxis to Instruct Regeneration - 2023.12.10.570982v1.full.pdf
www.biorxiv.org
Scx expression begins in activated SCs and continues throughout regenerative myogenesis after injury. By SC-specific Scx gene inactivation (ScxcKO), we show that Scx function is required for SC expansion/renewal and robust new myofiber formation after injury. In adult muscles, Hic1+ quiescent mesenc
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  • Scx expression begins in activated SCs and continues throughout regenerative myogenesis after injury.
  • By SC-specific Scx gene inactivation (ScxcKO), we show that Scx function is required for SC expansion/renewal and robust new myofiber formation after injury.
  • In adult muscles, Hic1+ quiescent mesenchymal progenitors (MPs) give rise to Scx+ cells in the muscle interstitial compartment, and ablation of Hic1+ cells negatively impacts muscle regeneration18.
  • Muscle interstitial Scx+ cells engrafted into the muscle contribute only to extracellular matrix remodeling19. A survey of muscle interstitial CT assigned a sub-population of cells expressing tendon markers (including Scx) as paramysial cells - cells lining next to the perimysium that wraps around muscle fascicles20. Furthermore, Strenzke and colle...
  • we utilized ScxCreERT2 for tamoxifen (TMX) inducible lineage tracing with a tdTomato (tdT) reporter (RtdT)24. Two experimental groups with different tamoxifen (TMX) and injury regimen were designed (Fig. 2A): 1) TMX-induced marking before injury, and 2) TMX-induced marking after injury; muscles were harvested at 14 dpi for analysis.

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scx
satellite cells
skeletal muscle
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