High coverage of single cell genomes by T7-assisted enzymatic methyl-sequencing
www.biorxiv.org
However, most of these approaches ignored the amplification bias of bisulfite-treated DNA, which leads to shallow genome coverage. T7-assisted enzymatic methyl-sequencing method (TEAM-seq) he standard and well-established technology to detect sequence-specific 5mC marks is bisulfite sequencing The s
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- However, most of these approaches ignored the amplification bias of bisulfite-treated DNA, which leads to shallow genome coverage.
- T7-assisted enzymatic methyl-sequencing method (TEAM-seq)
- he standard and well-established technology to detect sequence-specific 5mC marks is bisulfite sequencing
- The standard and well-established technology to detect sequence-specific 5mC marks is bisulfite sequencing 6, 7, which utilizes sodium bisulfite to convert the unmethylated cytosine to uracil after constructing the sequencing library
- Methylated cytosine is resistant to bisulfite conversion
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