The UBR box E3 ligases Poe and Hyd are required for efficient Pericentrin degradation thumbnail
The UBR box E3 ligases Poe and Hyd are required for efficient Pericentrin degradation
www.biorxiv.org
Both IP and Y2H revealed that the C-terminal catalytic domain of Hyd, containing the Hect substrate recognition domain (aa 2484-2885), bound directly to each of PLPF2 and PLPF3 (Figure 7C, D, F). Additional Y2H experiments using the C-terminal end of PLPF5 (aa 2539-2895) revealed direct interaction
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  • Both IP and Y2H revealed that the C-terminal catalytic domain of Hyd, containing the Hect substrate recognition domain (aa 2484-2885), bound directly to each of PLPF2 and PLPF3 (Figure 7C, D, F).
  • Additional Y2H experiments using the C-terminal end of PLPF5 (aa 2539-2895) revealed direct interaction with the conserved ZnF of PoeF2 (aa 1741-1920; Figure S4, row 6), but we did not pursue this interaction further.

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