Both IP and Y2H revealed that the C-terminal catalytic domain of Hyd, containing the Hect substrate recognition domain (aa 2484-2885), bound directly to each of PLPF2 and PLPF3 (Figure 7C, D, F).
Additional Y2H experiments using the C-terminal end of PLPF5 (aa 2539-2895) revealed direct interaction with the conserved ZnF of PoeF2 (aa 1741-1920; Figure S4, row 6), but we did not pursue this interaction further.
Glasp is a social web highlighter that people can highlight and organize quotes and thoughts from the web, and access other like-minded people’s learning.