We downloaded all genomes with an assembly status of “Complete genome” or “Chromosome” or “Scaffold” generated by the HMP from the GenBank database
The Wilcoxon rank-sum tests (two-sided) to assess the significance of the differentiations between metagenomes from different groups (i.e., water layers or body sites), were performed with the wilcox.test function from the R package stats
Capitalizing on the fact that BGC domains can be readily annotated in unassembled metagenomic data, and used to identify the different natural product BGC classes, we developed BiG-MEx
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