Base-edited CAR T Cells for combinational therapy against T cell malignancies thumbnail
Base-edited CAR T Cells for combinational therapy against T cell malignancies
www.biorxiv.org
High level surface disruption of TCRαβ/CD3 and CD7 by flow cytometry (63.7% and 60.8%, respectively) was achieved using SpCas9 mRNA and was associated with robust CAR expression throughout culture (78.2% 3CAR+ and 80.5% 7CAR+) Reduced TCR/CD3 and CD7 expression in edited groups and high level CAR ex
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  • High level surface disruption of TCRαβ/CD3 and CD7 by flow cytometry (63.7% and 60.8%, respectively) was achieved using SpCas9 mRNA and was associated with robust CAR expression throughout culture (78.2% 3CAR+ and 80.5% 7CAR+)
  • Reduced TCR/CD3 and CD7 expression in edited groups and high level CAR expression with fratricide evasion
  • Self-enrichment effects followed co-culture of 3CAR and 7CAR products (n=2) resulted in enriched TCR-CD7- 3CAR/7CAR cells (red box).
  • Cytotoxic activity was comparable to spCas9-edited 3CAR or 7CAR cells indicating intact functional integrity after base editing

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