sensitive and reproducible across a large variety of targets, the accuracy of DNA interrogation and subsequent enzyme activity is extremely critical, particularly when clinical decisions are to be made based on these results
substrate cleavage mediated by an active CRISPR ribonucleoprotein (RNP)
binding through a catalytically inactive RNP complex.
Among the CRISPR proteins that have been used so far, Cas9 and Cas12 or their inactive forms have been predominantly employed for detecting DNA sequences while Cas13 has been used for both DNA and RNA sequences.
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