Dynamics of transcriptional programs and chromatin accessibility in mouse spermatogonial cells from early postnatal to adult life
elifesciences.org
Overall, the technical aspects of the sequencing analyses and computational/bioinformatics seem sound but there are several concerns with the cell population isolated from testes There appears to be a lack of basic knowledge of the process of spermatogenesis. For instance, the statement that "During
1 Users
0 Comments
9 Highlights
0 Notes
Top Highlights
- Overall, the technical aspects of the sequencing analyses and computational/bioinformatics seem sound but there are several concerns with the cell population isolated from testes
- There appears to be a lack of basic knowledge of the process of spermatogenesis.
- For instance, the statement that "During the first week of postnatal life, a population of SCs continues to proliferate to give rise to undifferentiated Asingle (As), Apaired (Apr) and Aaligned (Aal) cells. The remaining SCs differentiate to form chains of daughter cells that become primary and secondary permatocytes around postnatal day (PND) 10 t...
- It is not clear which spermatogonial subtype the authors intended to profile with their analyses
- On the one hand, they used PLZF to FACS sort cells. This typically enriches for undifferentiated spermatogonia.
Domain
Ready to highlight and find good content?
Glasp is a social web highlighter that people can highlight and organize quotes and thoughts from the web, and access other like-minded people’s learning.