Distinct states of nucleolar stress induced by anti-cancer drugs
elifesciences.org
For a readout, we developed a novel parameter termed “nucleolar normality score”, which measures ratios of dense fibrillar center and granular component in the nucleolus and nucleoplasm. CDK9, the main CDK that targets RNA Pol II. Inhibition of CDK9 dramatically reduced rRNA production, caused disso
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- For a readout, we developed a novel parameter termed “nucleolar normality score”, which measures ratios of dense fibrillar center and granular component in the nucleolus and nucleoplasm.
- CDK9, the main CDK that targets RNA Pol II. Inhibition of CDK9 dramatically reduced rRNA production, caused dissociation of RNA Polymerase I catalytic subunit POLR1A from ribosomal DNA and dispersal of the nucleolar granular component, a stress we refer to as the “bare scaffold” state
- molecular dynamics analysis of the endogenous nucleolar protein NPM1 demonstrated that CDK inhibition vastly increased its mobility, consistent with the loss of nucleolar integrity
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rDNA
nucleolus
Treacle
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Toughts & Comments
Y-T Tsai
DRB, CK2 inhibitor?
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