An optimized CRISPR/Cas9 approach for precise genome editing in neurons
elifesciences.org
Through targeting non-coding regions TKIT is resistant to INDEL mutations. Utilizing in utero electroporation or viral injections in mice TKIT can label AMPAR subunits with Super Ecliptic pHluorin, enabling visualization of endogenous AMPARs in vivo using two-photon microscopy.
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- Through targeting non-coding regions TKIT is resistant to INDEL mutations.
- Utilizing in utero electroporation or viral injections in mice TKIT can label AMPAR subunits with Super Ecliptic pHluorin, enabling visualization of endogenous AMPARs in vivo using two-photon microscopy.
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