Landscape mapping of shared antigenic epitopes and their cognate TCRs of tumor-infiltrating T lymphocytes in melanoma
elifesciences.org
multimer-positive antitumor T cells were collected and their TCR genes were molecularly cloned. The antigen specificity and functional reactivity of the cloned TCRs were verified by multimer staining and ELISPOT assays of TCR-reconstituted T cells.
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- multimer-positive antitumor T cells were collected and their TCR genes were molecularly cloned.
- The antigen specificity and functional reactivity of the cloned TCRs were verified by multimer staining and ELISPOT assays of TCR-reconstituted T cells.
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