An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms
elifesciences.org
ingle stranded homology donors typically rely on much shorter homology arms (50–100 nt) to successfully integrate short DNA segments in Drosophila (~200 nt) based on homology-directed repair (Beumer et al., 2013; Gratz et al., 2013; Wissel et al., 2016; Bier et al., 2018).
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- ingle stranded homology donors typically rely on much shorter homology arms (50–100 nt) to successfully integrate short DNA segments in Drosophila (~200 nt) based on homology-directed repair (Beumer et al., 2013; Gratz et al., 2013; Wissel et al., 2016; Bier et al., 2018).
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