PPM1H phosphatase counteracts LRRK2 signaling by selectively dephosphorylating Rab proteins
elifesciences.org
In this study, we set out to identify and characterize the protein phosphatase(s) that counteract LRRK2 signaling by dephosphorylating LRRK2-modified Rab proteins. basal phosphorylation of Rab10 (Thr73) monitoring Thr73 Rab10 phosphorylation is currently the most facile readout of LRRK2 kinase activ
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- In this study, we set out to identify and characterize the protein phosphatase(s) that counteract LRRK2 signaling by dephosphorylating LRRK2-modified Rab proteins.
- basal phosphorylation of Rab10 (Thr73)
- monitoring Thr73 Rab10 phosphorylation is currently the most facile readout of LRRK2 kinase activity.
- The ratio of phospho-Thr73 Rab10 versus total Rab10 was used to calculate the degree of Rab10 phosphorylation in each cell lysate
- The top hits that enhanced Rab10 protein phosphorylation over 1.5-fold in Screen 1 and 2 were PPM1H (Metal-dependent protein phosphatases [PPM] family serine/threonine phosphatase)
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