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• electrophysiological function (Eugène et al., 2014) and optogenetic targeting (Andersson et al., 2016) in human brain slice cultures. Subsequent studies established successful neuronal labeling, optical manipulation and calcium imaging by virus-driven rapid expression of respective probes in short term cultures of several days (Ting et al., 2018).
• up to three weeks under specified conditions
• Recordings of pyramidal neurons showed a typical regular spiking pattern in acute and cultured slices (Figure 1B,C), as described in previous studies investigating human pyramidal neurons in acute slices (Verhoog et al., 2013).
• There was no significant correlation between the combined dendritic length and the DIV, neither for GFP-based tracings (data not shown) nor for analysis after pooling all biocytin and GFP reconstructed neurons (Figure 5C, middle panel).
• we found no significant changes of the absolute numbers of double-positive neurons in slice cultures between 0 DIV (3 slices), 9 DIV (3 slices) and 14 DIV (2 slices) (Dunn's multiple comparisons test, Figure 1—figure supplement 1B).

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