Single-color, ratiometric biosensors for detecting signaling activities in live cells
elifesciences.org
that only occupy a single channel Hence, rather than using the emission ratio between the FRET and donor channels, we instead used the loss of polarization of emitted light as a readout for FRET between two fluorescent proteins (FPs)
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- that only occupy a single channel
- Hence, rather than using the emission ratio between the FRET and donor channels, we instead used the loss of polarization of emitted light as a readout for FRET between two fluorescent proteins (FPs)
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