Islets were harvested and fixed in 2.5% polyformaldehyde and glutaraldehyde in PBS with a pH of 7.4 at room temperature for 1 hr. Then, samples were post-fixed with 1% osmium tetroxide in 0.1 mol/L sodium cacodylate for 1.5 hr, and stained with 1% (w/v) uranyl acetate in double distilled water for 50 min to increase the contrast under SEM. After wa...
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