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• Second, the ultrastructural features of several sensory cilia have remained poorly described, in part due to limitations in the visualization methods, but also due to issues arising from conventional chemical fixation of biological structures. For instance, the morphology and ultrastructure of cilia in the BAG gas-sensing and FLP nociceptive neuron...
• This analysis has now allowed us to robustly model the morphologies of all anterior sensory cilia, comprising 50 of 60 ciliated neurons in the C. elegans adult hermaphrodite
• Moreover, this high-resolution map reveals three distinct and novel MT distribution processes in branched cilia, suggesting diverse modes of ciliary protein transport and delivery.
• In particular, we noted that: (a) subcellular structures in muscle were maintained, allowing resolution of the paracrystalline organization of actin-myosin fibers in sarcomeres (Figure 1—figure supplement 1A); (b) cell–cell connections as in the electron-dense C. elegans apical junctions (CeAJs) (Labouesse, 2006; Lynch and Hardin, 2009) or hemi-des...
• (c) ciliary membranes exhibited well-defined, smooth structures with clearly resolved bilayers as compared to scalloped membranes observed in chemically fixed samples

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